Lipid Composition of Chlamydia psittaci Growth in Monkey Kidney Cells in Defined Medium

Improved chemically defined basal medium (CMRL-1969) for primary monkey kidney and human diploid cells.

An improved tissue culture basal medium, CMRL-1969, supplemented with serum, has been evaluated by measuring the growth responses of primary cultures of trypsin-dispersed monkey kidney cells (PMKC) and of an established culture of a human diploid cell strain (HDCS). Medium H597, an early modification of medium 199 which has been used successfully in the preparation of poliomyelitis vaccine for ...

Lipid Composition of the Hemagglutinating Active Fraction Obtained from Chick Embryos Infected with Chlamydia psittaci 6BC.

The lipid composition of the concentrated hemagglutininating active fraction (HF) of allantoic fluid from infected eggs, but free from Chlamydia psittaci 6BC, was compared to concentrated normal allantoic fluid (NAF). Phosphatidyl-choline (PC) and phosphatidylethanolamine were the major lipid classes of the total phospholipid fraction. Some quantitative differences were found in the amount of P...

Growth of African Horse Sickness Virus in Monkey Kidney Cell Cultures

Growth of NS0 cells in protein-free, chemically defined medium.

Many hybridoma and recombinant myeloma cell lines have been successfully adapted to growth in protein-free media. Compared with serum-supplemented media, use of protein-free media promotes superior cell growth and protein expression and facilitates downstream purification of the expressed product. Owing to its sterol auxotrophy, the NS0 myeloma is normally grown in either a serum-supplemented m...

Sequential cellular changes produced by african horse sickness virus in monkey kidney cells